东北棕壤长期不同施肥处理轮作大豆氮素吸收和土壤硝态氮特征

【目的】在玉米–玉米–大豆轮作体系下,基于棕壤肥料长期定位试验,研究不同施肥处理对东北地区大豆生物量、产量、各部位吸氮量及收获期土壤0―100 cm硝态氮累积的影响,为该地区合理施肥提供理论依据和科学指导。【方法】棕壤肥料长期定位田间试验始于1979年,包括不施肥(CK)、单施氮肥(N)、氮磷钾肥配施(NPK)、低量厩肥(M1)及其与化肥配施(M1N和M1NPK)、高量厩肥(M2)及其与化肥配施(M2N和M2NPK)9个处理。厩肥为猪厩肥,1992年后大豆季不施猪厩肥,仅在玉米季相关处理中施用。39年后,调查分析了大豆生物量、产量、氮素吸收利用及大豆收获期0―100 cm土壤硝态氮累积特征。【结果】高量、低量厩肥配施化肥处理大豆生物量、产量、总吸氮量及各部位吸氮量均显著高于单施氮肥和不施肥处理,其中,M1NPK处理大豆生物量、产量和总吸氮量最高,分别为9107、2979和314.2 k g/h m^2,较其他处理分别提高了6.1%~133.6%、23.9%~232.5%和11.7%~359.4%。施肥提高了大豆氮收获指数,但氮素生理效率降低。NPK和M1NPK处理的氮素收获指数最高,均为63.5%,而氮素生理效率较CK分别降低了30.6%和28.1%。大豆收获期各处理土壤硝态氮累积量随土层深度的增加而降低。与播前相比,大豆收获期单施氮肥处理的0―100 cm土层硝态氮积累量显著增加,NPK处理变化不显著,M1、M1N和M1NPK处理显著降低。低量厩肥配施化肥处理收获期0―100 cm土壤硝态氮积累量远低于高量厩肥配施化肥处理,较播前平均降低了79.2%。所有处理中,土壤硝态氮积累量以M1NPK处理最低,比其他处理平均降低了58.2%。【结论】在东北棕壤地区玉米–玉米–大豆轮作体系下,玉米季低量厩肥(13.5 t/hm^2)与氮磷钾化肥配合施用时,大豆季仅施氮磷钾化肥既可提高大豆生物量、产量,促进氮素吸收,同时还可降低大豆收获期土壤硝态氮累积量,降低环境风险,是该轮作体系较为合理的施肥方式。     

1株H1N1亚型猪流感病毒的全基因组特征及其对小鼠的致病性

旨在了解河南省猪流感病毒的流行情况及其遗传进化和基因组特征。2018年4月，从河南省某一出现疑似流感症状猪群中采集鼻拭子样品150份用于分离病毒，对分离病毒的全基因组进行序列测定和分析。同时感染6周龄BALB/c小鼠，研究其对小鼠的致病性。结果显示，获得1株H1N1亚型病毒[命名为A/swine/Henan/NY20/2018（H1N1）]。遗传进化表明，其HA和NA基因属于欧亚类禽H1N1分支，PB2、PB1、PA、NP和M基因属于2009甲型H1N1分支，NS基因属于经典H1N1分支。HA蛋白的裂解位点序列为PSIQSR↓GL，具有低致病性流感病毒的分子特征，在小鼠肺和鼻甲有效复制并能引起肺组织病理学变化。本研究分离到1株3源重排H1N1亚型病毒，对小鼠呈现一定致病力，提示应进一步加强对SIV的监测。     

Artificial Feeding and Laboratory Rearing of Endangered Saproxylic Beetles as a Tool for Insect Conservation

Conservation of threatened animals is frequently limited by lack of knowledge about their ecological preferences, and often artificial feeding is one of the few chances to save endangered species. We investigated the possibility to artificially feed two endangered flat bark beetles dependent on dead wood for their diet—namely, Cucujus cinnaberinus (Scopoli, 1763) and Cucujus haematodes Erichson, 1845—by examining their dietary preferences, life cycle duration, and survival in laboratory conditions. Individuals of the two species were caught in the wild and larvae and adults were fed in laboratory conditions by live or dead prey. Three species of saproxylic beetles: two cerambycids (Acanthocinus griseus Fabricius, 1793 and Rhagium inquisitor Linnaeus, 1758), one scolytid (Ips sexdentatus Börner, 1776) one tenebrionid (Tenebrio molitor (Linnaeus, 1758)) one dipteran (Lucilia sericata (Meigen, 1826)) and one ant (Lasius sp. Fabricius, 1804) were used as prey, with minced meat as a control. Our results indicated high survival and no difference in prey choice between the two flat beetle species. Larvae and adults preferred dead prey, but no significant preference was detected among dead prey taxa, supporting the hypothesis that the two species are opportunistic scavengers. Comparing data with previous results, both species and their developmental stages should be classified as obligate saproxylic organisms with preference to the dead and decaying organic material. Successful artificial feeding and rearing of these endangered species, followed by the release in the wild through rescue or reintroduction programs, therefore appear relevant for their protection and future conservation.     

Screening of diverse cassava genotypes based on nitrogen uptake efficiency and yield

As one of the top three tuber crops of the world, cassava is a staple food and feed crop for tropical and subtropical regions. Because of its high drought resistance and tolerance to nutrient deficiency, cassava is usually cultivated on hilly areas that are nutrient-poor. Nitrogen(N) is one of the significant factors affecting cassava yield. A double factorial(N level×genotypes) split-plot field experiment was conducted to analyze differences in yield and N accumulation of 25 cassava genotypes under low-N conditions to screen for cassava genotypes with high-N efficiency. The two-year field experiment showed that cassava yield and N accumulation are determined by specific genotypes, soil N levels, and year. Among these factors, soil N levels are the main factors that are responsible for differences in cassava yield. When yield and relative N accumulation under low-N conditions were used as screening markers, we identified an efficient and responsive genotype(SC10), and two inefficient and non-responsive genotypes(SC205 and GR5). The efficient and responsive genotype and the inefficient and non-responsive genotype can be used as study materials to further reveal the mechanisms for high-N efficiency in cassava.     

Effects of bioflocs with different C/N ratios on growth,immunological parameters,antioxidants and culture water quality in Opsariichthys kaopingensis Dybowski

Biofloc technology (BFT) is a new ecological aquaculture technology that is intended not only to eradicate pollutants and elevate feed utilization but also to enhance immunity and antioxidant activity in aquatic animals. A 28‐day feeding trial was implemented to evaluate the effects of promoted bioflocs on the water quality, growth performance, immunological parameters and antioxidant status of Opsariichthys kaopingensis Dybowski juveniles in low exchange culture tanks. Three hundred sixty healthy O. kaopingensis juveniles (7.1 ± 0.02 g) were irregularly distributed among 12 tanks. Four C:N ratios in triplicate tanks were tried: C/N = 10.8:1 with a commercial diet (control), C/N = 15:1, C/N = 20:1 and C/N = 25:1. Anhydrous glucose (99.97%) was added to the commercial diet to maintain elevated carbon ratio in the three treatments. Uninterrupted 24‐hr aeration was supplied during the test by using an air‐stone connected to an air pump to develop the bioflocs in the water column. The results indicated that BFT significantly reduced total ammonia nitrogen (TAN), nitrite (NO2^?‐N), total phosphorus (TP) concentrations and water transparency (Secchi depth) in C/N 15, C/N 20 and C/N 25 (p < .05), whereas the chemical oxygen demand (COD) and nitrate nitrogen (NO3^?‐N) concentrations gradually increased over time. In addition, weight gain, specific growth rate (SGR), survival and protein efficiency ratio (PER) were significantly elevated in C/N 20, while food conversion rate (FCR) was considerably decreased (p < .05) in C/N 20, compared with the control. Significant increases in lysozyme (LSZ), glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and alkaline phosphatase (AKP) activities of O. kaopingensis were found in the C/N 20 group after a 28‐day feeding trial (p < .05). Comparing the antioxidant capacity of O. kaopingensis in gills, brains, kidneys, hepatopancreas, intestines and serum of juveniles from the four experimental groups, the activity of superoxide dismutase (SOD), total antioxidant capacity (T‐AOC), catalase (CAT) activity and glutathione peroxidase (GSH‐PX) activity of juveniles in the C/N 20 group were significant higher (p < .05), and the content of malondialdehyde (MDA) was considerably lower, than in the control. Overall, these findings suggest that BFT not only can enhance O. kaopingensis growth performance and strengthen antioxidant status but also can upgrade immune response, ensuring the sustainable development of aquaculture.     

林木氮素吸收偏好性及其形成机制研究进展

森林土壤中可被林木吸收利用的氮（N）素主要以铵态氮（NH₄+-N）和硝态氮（NO₃--N）的形态存在。受全球气候变暖、氮沉降和人类活动等因素的影响，NH₄+和NO₃-的分布存在很大的时间波动性和空间异质性，且NH₄+-N和NO₃--N亏缺已成为限制林地生产力的主要因素。在森林土壤N亏缺和N异质分布的逆境中，在林木长期进化过程中形成了对不同形态N素的吸收偏好，且这种吸收偏好会随生长环境条件而发生改变。特别是对于NH₄+和NO₃-这2种主要形态的偏好选择性已被证明是决定林木生产力、竞争、共存和生态演替的重要因素之一。对不同树种在N异质分布环境下的N吸收偏好和形成机制的研究，是揭示林木N素营养遗传特性和提高林地N素利用效率的关键。文中从森林土壤中N的主要形态及其分布特征、林木对不同形态N素的吸收偏好和形成机制、林木N吸收偏好的影响因素3个方面进行总结阐述，并对未来研究方向进行展望，以期为我国人工林培育中不同树种的造林配置和合理N素施肥技术提供理论依据和参考。     

Biochar addition mitigates nitrogen loss induced by straw incorporation and nitrogen fertilizer application

Biochar has been shown to be potentially beneficial for enhancing yields and soil properties, and diminishing nitrogen (N) losses. However, it remains unclear how biochar regulates soil carbon (C) and N to mitigate N losses induced by straw mixing with N fertilizer in dryland soils. Therefore, we investigated the effects of straw mixing (S₁), S₁ with biochar (SB) and no straw inputs (S₀), and routine urea application rates (N₁) and 70% of routine rates (N_0.7) on yields and N losses, and identify the relationship between N losses and soil C and N compounds. Results showed that N_0.7 and N₁ were suitable for the maize and wheat seasons, respectively, contributing to mitigating N losses without reducing crop yields. Moreover, in the maize season, N_0.7-SB significantly mitigated the straw-induced NH₃-N and N₂O-N emissions by 106% and 81%, respectively. In the wheat season, N₁-SB reduced the straw-induced NH₃-N and N₂O-N emissions by 35% and 66%, respectively. In addition, N_0.7-SB sharply reduced soil inorganic N (SIN) storage in the maize season. Furthermore, the NH₃-N and N₂O-N emission rates were negatively correlated with dissolved organic carbon/SIN content (0–20 cm) (DOC/SIN_0-20). N losses (N₂O-N and NH₃-N emissions and SIN storage) were positively correlated with SIN_0-20, but negatively correlated with soil organic carbon / SIN_0-20 (SOC/ SIN_0-20). This study provides further evidence that biochar with an appropriate N application rate decreased SIN_0-20 and increased DOC/SIN_0-20, thus reducing SIN storage and the straw-induced gaseous N emissions without decreasing crop yields.     

稻田转为菜地初始阶段温室气体排放特征

【目的】近年来,随着我国社会经济的快速发展和人们生活水平的提高及膳食结构的改善,越来越多的稻田被转为蔬菜种植,影响了土壤碳氮转化过程及其引起的温室气体排放。因此有必要探究稻田转为蔬菜种植,特别是该土地利用方式转变初始阶段的温室气体（CH₄和N₂O）排放特征及其关键影响因素。【方法】试验选取了长期种植水稻的双季稻田,将其中一部分转为蔬菜种植,另一部分继续种植水稻,每个处理设置了3个重复,按照当地常规模式进行管理。采用静态暗箱—气相色谱法连续3年进行田间原位观测,比较分析稻田和由稻田转变的菜地CH₄和N₂O排放特征及其年际变化差异,明确稻田转为菜地初始阶段CH₄和N₂O排放的关键影响因素。【结果】稻田是重要的CH₄排放源,其第一年的排放强度（183.91 kg CH₄-C·hm^-2?a^-1）明显低于后续两年（241.56—371.50 kg CH₄-C·hm^-2?a^-1）,这主要归功于后两年降雨量的增加引起了土壤水分含量的升高。稻田转为菜地显著减少了CH₄排放,减少量相当于稻田CH₄年累积排放量的83%—100%。菜地第一年的CH₄累积排放量（31.22 kg CH₄-C·hm^-2）显著高于第二年（0.45 kg CH₄-C·hm^-2）和第三年（0.89 kg CH₄-C·hm^-2）,表明稻田转菜地对CH₄排放的影响具有时间滞后效应。稻田是弱的N₂O排放源（1.35—3.49 kg N₂O-N·hm^-2?a^-1）,其转为菜地显著增强了N₂O排放。菜地第一年的N₂O累积排放量（95.12 kg N₂O-N·hm^-2）显著高于第二年（38.28 kg N₂O-N?hm^-2）和第三年（40.07 kg N₂O-N·hm^-2）。菜地土壤异养呼吸对N₂O排放的影响在第一年明显高于第二、三年,表明稻田转为蔬菜种植的第一年,有机质矿化对N₂O排放有重要贡献。在100年尺度CO₂当量下,稻田转为蔬菜种植第一和第二年的综合增温潜势（GWP）相对于稻田分别显著增加了390%和98%,主要是由于增加的N₂O增温潜势超过了减少的CH₄增温潜势。但是,稻田转为菜地的第三年,菜地的GWP（(16.72±3.25) Mg CO₂-eq·hm^-2）与稻田（(14.84±1.39) Mg CO₂-eq·hm^-2）相比无显著差异,主要是由于减少的CH₄ 增温潜势完全抵消了增加的N₂O增温潜势。这些研究结果表明稻田转菜地对GWP的影响主要集中在该土地利用方式转变的第一年。【结论】稻田转为菜地显著减少了CH₄排放,增加了N₂O排放,增强了菜地第一和第二年的综合增温潜势。有机质矿化过程对新转菜地第一年较高的N₂O排放有重要贡献。这些研究结果表明了评价土地利用方式转变初始阶段温室气体排放特征的重要性,便于及时采取有效管理措施缓解温室气体排放,实现环境友好型农业可持续生产。     

温室茄子氮、磷、钾素最大利润施用量研究

为了研究温室茄子氮、磷、钾素最大利润施用量,为茄子合理施肥和安全生产提供参考,在甘肃省张掖市甘州区党寨镇温室内,采用田间小区试验方法,进行了温室茄子氮、磷、钾素最大利润施用量研究。结果表明:随着氮、磷、钾素施用量梯度的增大,茄子产量逐渐增加,边际产量、边际产值和边际利润均呈递减趋势,其中边际利润最终出现负值。根据最大利润施用量计算公式求得茄子氮、磷、钾素最大利润施用量分别为0.62、0.41、0.29 t/hm~2,进而得出最大利润施用量的理论产量分别为95.58、96.04、95.35 t/hm~2。     

无机碳源对零价铁介导的自养脱氮序批式反应器处理高氨氮废水的影响

无机碳源作为自养微生物的能量来源,是影响自养脱氮细菌富集的重要因素。文章通过添加不同量的KHCO3作为ZVI介导的自养脱氮体系中无机碳源,研究反应体系的脱氮效果影响趋势以及适宜的无机碳源添加量。结果如下:1)KHCO3作为无机碳源可以显著提高NH^+4-N去除率,KHCO3添加量越多,其NH^+4-N去除率越高。KHCO3添加量分别为2 g,1 g,0.5 g的SBR反应器R2,R1,R0.5的NH^+4-N去除率分别为98.39%,65.18%,44.56%,相较不添加KHCO3的R0分别提高86.5%,53.29%,32.67%。ZVI的添加会降低KHCO3对NH^+4-N氧化的促进作用。2)KHCO3可明显提高TIN去除效果,促进总氮脱除的高低顺序是R1>R0.5>R2,SBR反应器R2,R1,R0.5的平均TIN去除率分别为19.01%,32.04%,27.62%,相较于空白组R0分别提高了10.60%,23.63%,19.21%。3)KHCO3可中和硝化反应产生的H^+,对反应体系具有缓冲作用,可使微生物处于较适宜的酸碱环境中,更有利于反应器的稳定运行。4)适量的无机碳源KHCO3可以促进氨氧化细菌和厌氧氨氧化细菌的活性。该研究探讨了无机碳源在零价铁脱氮体系中的影响趋势,为铁型脱氮技术提供了理论支撑。